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Journal of Stem Cell Research and Tissue Engineering
Published by Universitas Airlangga
ISSN : 26141264     EISSN : 26141256     DOI : https://dx.doi.org/10.20473/jscrte
Core Subject : Health, Science, Engineering,
Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary
Journal of Stem Cell Research and Tissue Engineering (JSCRTE) is published by Stem Cell Research and Development Center, Airlangga University. Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancerstem cells, developmental studies, genomics and translational research. Special focus of JSCRTE is on mechanisms of pluripotency and description of newly generated pluripotent stem cell lines. Articles that go through the selection process will be review by peer reviewer or editor. The journal is published regularly twice a year in December and May. Every publication consists of 60-70 pages and 5 scientific articles in the form of research, study literature, and the case study in English. The contributors Journal of Stem Cell Research and Tissue Engineering are Stem Cell researchers, lecturers, student and practitioners that came from Indonesia and abroad.
Arjuna Subject : Kedokteran - Imonologi dan Alergi
Articles 5 Documents
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Search results for , issue "Vol. 6 No. 1 (2022): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING" : 5 Documents clear
Effect of Concentration of Mangrove Leaf Extract Lumnitzera Racemosa on Hela Cell Viability Ayu Kartika Fitri Ayu
Journal of Stem Cell Research and Tissue Engineering Vol. 6 No. 1 (2022): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v6i1.37511

Abstract

Cervical cancer is a disease caused by a malignant process that occurs in the cervix or cervix. The cause of cervical cancer is not known for certain, but it is estimated that around 95% is caused by HPV (Human Papilloma Virus). Efforts to cure cancer with drugs (pharmacotherapy) or with chemical compounds (chemotherapy) in general have not been able to give satisfactory results, so alternative treatment methods are sought, including traditional medicine, namely by using mangroves. Lumnitzera racemosa is one type of mangrove plant that has been used in alternative medicine because of its potential as anticancer. The aim of this study was to determine the effect of Lumnitzera racemosa mangrove extract on hela cell viability. Lumnitzera racemosa leaf powder was extracted using graded maceration. The solvents used include n-hexane, ethyl acetate, and ethanol. The results showed that the LC50 value was 56 ppm, it means that the ethanol extract has toxic properties. The results of the phytochemical test of the leaf extract of Lumnitzera racemosa contained alkaloids, steroids, triterpenoids and saponins. The test results showed that the extract yield was 11.58%, the water content of the extract was 22.17%, and the total phenol was 2742.17 mg GAE. The test results from the LC-MS test resulted in suspected compounds including pyrogallol, isoniazid and caffeine. The ethanolic extract of Lumnitzera racemosa leaf was cytotoxic to the viability of hela cells with the resulting IC50 value of 493.33 µg/mL.
Levels of Wistar Calcium Serum (Rattus Norvegicus) in Human Adipose-Derived Mesenchymal Stem Cells (hADMSCs) and Chitosan Scaffold by Osteoinduction Examination Destri Imania Destri
Journal of Stem Cell Research and Tissue Engineering Vol. 6 No. 1 (2022): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v6i1.37512

Abstract

Bone tissue reconstruction with extensive damage is one of the challenges for dentists because its healing process of bone tissue. Bone graft is the gold standard for bone repair. However, the use of bone graft has a limited amount of tissue produced. Tissue engineering is the latest method in terms of bone regeneration. Tissue engineering has three main components, first is stem cells that have self renewal ability and multineage differentiation, second is bioreactor / growth factor, and then scaffold. The combination of hADMSC and chitosan scaffold, is expected to trigger osteoinduction shown by osteogenic markers such as calcium levels. Purpose to prove osteoinduction in a combination of Human Adiposed Derived Mesenchymal Stem Cell (hADMSC) and chitosan scaffold using blood serum calcium levels. Methods: This study uses 12 treatment groups with each group having 4 samples. Groups 1 to 4 were the negative control group at 1st,3rd,7th, and 14th days which maxillary bone drilling only. While groups 5 to 8 were the positive control group at 1st,3rd,7th, and 14th days which were given chitosan scaffold. Groups 9 to 12 were treatment group at 1st,3rd,7th, and 14th days which were given hADMSC and chitosan scaffold. Blood collection is carried out in each group to check serum calcium levels. Result there were differences in calcium levels in blood serum in each group. Conclusion the application of hADMSC and chitosan scaffold caused a significant change in serum calcium levels on the 1st, 3rd, 7th and 14th days which meant that the combination of hADMSC and chitosan scaffold could trigger osteoinduction.  
The Effect of Human Adipose-Derived Mesenchymal Stem Cell (HADMSC) With Chitosan Scaffold on Bone Defect White Rats (Rattus Norvegicus) on Serum Alkaline Phosphatase (ALP) Levels Fauzan Fauzan
Journal of Stem Cell Research and Tissue Engineering Vol. 6 No. 1 (2022): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v6i1.37514

Abstract

Bone defect is one of the challenges for dentists in the process of healing bone tissue. Bone defect can occur in alveolar bone with the etiology of microorganisms and cyst expansion. In addition, cases of bone defects in alveolar bone are also often found in cases with treatment of apex resection and hemisection. Autologous bone graft is a clinical gold standard in the treatment of bone defect. However, the use of bone graft has a limited number of growth factors produced. Tissue engineering is the latest method in terms of bone regeneration. Tissue engineering has three main components; stem cell, growth factor, and scaffold. Stem cells will increase osteoblastogenesis and chitosan scaffold will immobilize alkaline phosphatase (ALP) so that serum ALP levels decrease and bone regeneration and mineralization processes become faster. The aim of this study is analyzing the effect of human adipose-derived mesenchymal stem cell (HADMSC) with chitosan scaffold (CS) in bone defect on serum alkaline phosphatase (ALP) levels. This research was a in vivo laboratory experimental study. Bone defects are planted with chitosan scaffold (CS) and a combination of human adipose-derived mesenchymal stem cells (HADMSC) with chitosan scaffold. Measurement of ALP levels was carried out by the International Federation of Clinical Chemistry (IFCC) method using an analyzer on the 1st, 3rd, 7th and 14th days. Research data were analyzed using multivariate analysis of variance (MANOVA) and Bonferroni tests. The results of the data analysis showed that there were significant differences in ALP levels with CS planting and the combination of HADMSC and CS. the effect of human adipose- derived mesenchymal stem cell (HADMSC) with chitosan scaffold (CS) on bone defect reduces serum alkaline phosphatase (ALP) levels on the 3th and 14th days.
Viabilty Test of Fish Scale Collagen (Oshpronemus Gouramy) on Bone Marrow Mesenchymal Stem Cell Culture Nur Azizah Hadi Azizah
Journal of Stem Cell Research and Tissue Engineering Vol. 6 No. 1 (2022): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v6i1.37515

Abstract

The role of type I collagen is as a matrix of extracellular proteins with characteristics of increased cell proliferation which directly affects the physiology and morphology of cells. Type 1 collagen can be obtained either from fish scales. This is what underlies the author to support engineering tissue used for the treatment of periodontal disease in the regenerative field by utilizing collagen derived from gouramy scales. As an initial step, the researchers wanted to conduct a study using collagen extract derived from gouramy scales (Osphoronemus gouramy) which was applied to bone marrow mesenchymal stem cell cultures to see viability in vitro. To determine the viability of collagen in carp (Osphronemus goramy) scales to bone marrow mesenchymal stem cells. Bone marrow mesenchymal stem cells are taken from mice and planted in 96 well plates. Collagen extracted from gouramy scales using the enzymatic method was dissolved in a condition medium and hydrolyzed into a collagen hydrolysis solution with each concentration of 0.01 mg / ml, 0.02 mg / ml, 0.04 mg / ml, 0.16 mg / ml, 0.32 mg / ml was put into the well prepared and incubated for 24 hours for the MTT assay. Collagen in carp scales can increase the viability of bone marrow mesenchymal stem cells with a percentage above 50% and the highest viability concentration at 0,01 mg / ml. The collagen of gouramy scales soaked in a medium condition has better viability than the collagen hydrolysis solution of carp. Collagen in carp scales is viable against bone marrow mesenchymal stem cells. Collagen scales of gouramy soaked in medium had the highest viability with an optimum dose of 0.01 mg / ml.
Viabilty Test of Fish Scales Collagen Gourami (Oshpronemus Gouramy) on Human Gingival Fibroblasts Cells Widya Rizky Romadhona Widya
Journal of Stem Cell Research and Tissue Engineering Vol. 6 No. 1 (2022): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v6i1.37516

Abstract

Periodontal disease is a pathological inflammatory condition of the periodontal tissues surrounding the teeth, including Human Gingival Fibroblasts (HGF) which is one of the major components of tissue formation in periodonsium. HGF regeneration with the accelerating proliferation of tissue engineering therapy needs. Generally, the tissue engineering using regenerative materials from cow or pig as the therapies, but these materials have some flaws so this research to find alternative materials regenerative tissue engineering scaffold collagen type 1 derived from fresh water fish scales, one of which are gourami fish scales. This research was conducted to test the viability of fish scales collagen gouramy against Human Gingival Fibroblasts for 24 hours. This study aimed to determine the concentration of fish scale collagen gourami which can maintain the viability of human gingival fibroblast cells for 24 hours. HGF is taken from healthy gingiva and planted in 96 well plates. Fish scales collagen gouramy with a concentration of 0.32 mg/ml, 0.16 mg/ml, 0.04 mg/ml, 0.02 mg/ml and 0.01 mg/ml were added to each well and incubated during 24 hours. MTT Assay is performed to see the viability of fibroblast cells. The viability of HGF were increased after the addition of fish scales collagen gourami on concentration 0.32 mg/ml until 0.01 mg/ml. The highest viability of the cells was shown after the addition of 0.01 mg/ml. Fish scales collagen gouramy has the potential in tissue engeneering and the concentration of 0.01 mg/ml shows the highest viability of HGF.

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